Journal: Turkish Journal of Chemistry

Article Title: Expression, purification, and crystallization of recombinant human ABL-1 kinase for X-ray crystallography

doi: 10.55730/1300-0527.3776

Figure Lengend Snippet: The SDS-PAGE analysis of the recombinant ABL-1 expression in E. coli Rosetta-II. M: Marker, BI: Before Induction, WC: Whole Cell Lysate, Sup: Supernatant, PD: Pull down sample.

Article Snippet: Plasmids were transformed into E. coli Rosetta2 BL21 competent cells using the heat shock method by applying heat at 42 °C for 45 s. The transformed cells were grown on Luria-Bertani (LB) agar plates containing 50 μg/mL kanamycin (Cat#KB0286, Bio Basic, Canada) and 35 μg/mL chloramphenicol (Cat#CB0118, Bio Basic, Canada) at 37 °C overnight.

Techniques: SDS Page, Recombinant, Expressing, Marker

Journal: bioRxiv

Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis

doi: 10.64898/2025.12.16.694762

Figure Lengend Snippet: (A) Schematic of MBP-SLC25A45-expressing E.coli and representative immunoblotting of MBP-SLC25A45. (B) AlphaFold structure (AF-Q3KQZ1-F1-model_v4) for SLC25A45. (C) Cartoon/ribbon representation of SLC25A45 prepared structure showing its nine (six in vertical – α1, α3, α4, α6, α7, and α9) transmembrane alpha helices and corresponding range of amino acids, in respective coloring scheme. (b) Surface and cartoon (in white) representation of SLC25A45 prepared structure and detected cavities (pockets) by Sitemap . (D) A 3D view of the docking pose was obtained for TML (orange) key interactions (dashed lines in yellow and magenta correspond to the hydrogen bond and salt bridge, respectively), which was established with SLC25A45 key residues (blue). (E) 2D interaction diagram for TML-SLC25A45, where magenta arrows and solid lines represent hydrogen bonds and salt bridges, respectively.

Article Snippet: Expression of MBP-hSLC25A45 was performed in E. coli C43(DE3) cells (Biosearch Technologies, CMC0019-20X40UL).

Techniques: Expressing, Western Blot

Journal: bioRxiv

Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis

doi: 10.64898/2025.12.16.694762

Figure Lengend Snippet: (A) Time-dependent D 9 -TML uptake in E. coli cells expressing an empty vector (control) and SLC25A45 fused in frame to MBP (MBP-SLC25A45) (n=4). (B) Substrate specificity of SLC25A45 in E. coli cells expressing MBP-SLC25A45 (n =5 for D 9 -TML and D 9 -γ-BB, n=4 for D 9 -choline). (C) Side view of SLC25A45 structure (AF-Q8N413-F1-v4; blue ribbons and surface) at the inner mitochondrial membrane. (D) Key amino acid residues (blue) at the central channel-like cavity. (E) Left: Side view of TML (orange carbons and surface) within the SLC25A45 binding cavity. Right: Top view of TML within the SLC25A45 binding cavity. (F) Close-up 3D view of TML (orange carbons in ball and stick) key interactions based on the MD replicate simulations. (G) Average RMSD against time plot obtained by MD simulations for SLC25A45 apo and complexed with TML. (H) Interaction bar chart for one of the TML-SLC25A45 MD replicate simulations. Bars represent mean and error, as shown in s.e.m. P values were calculated by two-way ANOVA with Šídák’s multiple comparisons test (A) or unpaired t-test (B).

Article Snippet: Expression of MBP-hSLC25A45 was performed in E. coli C43(DE3) cells (Biosearch Technologies, CMC0019-20X40UL).

Techniques: Expressing, Plasmid Preparation, Control, Membrane, Binding Assay